ZIA BC 011294 (ZIA) | |||
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Title | Conditional manipulation of hnRNPLL expression in murine models | ||
Institution | NCI, Bethesda, MD | ||
Principal Investigator | Oberdoerffer, Shalini | NCI Program Director | N/A |
Cancer Activity | N/A | Division | CCR |
Funded Amount | $73,430 | Project Dates | 10/01/2009 - 00/00/0000 |
Fiscal Year | 2014 | Project Type | Intramural |
Research Topics w/ Percent Relevance | Cancer Types w/ Percent Relevance | ||
Autoimmune Diseases (25.0%) Cancer (100.0%) |
Hodgkins disease (50.0%) Non Hodgkins Lymphoma (50.0%) |
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Research Type | |||
Normal Functioning Endogenous Factors in the Origin and Cause of Cancer |
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Abstract | |||
Mice for conditional hnRNPLL expression or deletion were generated. HnRNPLL cDNA was knocked into the ROSA26 locus downstream of a lox P flanked STOP cassette. These mice were crossed to mice with Cre-recombinase expression from the CD19 promoter, leading to hnRNPLL expression early in B cell development with the expected effect on CD45 alternative splicing. However, we were unable to observe a significant effect on B cell development. We are examining these mice in greater detail in collaboration with Dr. Anjana Rao of the La Jolla Institute. We have further generated mice for Cre-recombinase mediated hnRNPLL deletion. Similar to the CD19 knockin mice, deletion of hnRNPLL in B cells of CD19-Cre mice did not impact B cell development. However, preliminary data suggest that deletion of hnRNPLL in T cells of Lck-Cre mice perturbs early thymic development. We encountered breeding issues when the mice were moved into a new habitat and are currently working to regenerate our colonies. Our goal is to examine the mechanistic basis of altered T cell selection in response to hnRNPLL loss." |